pET-28a(+) Sequence and Map - SnapGene
WEBBacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28b(+) or pET-28c(+).
pET-28b(+) Sequence and Map - SnapGene
WEBBacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28a(+) or pET-28c(+).
Addgene: Vector Database - pET-28 a (+)
WEBAnalyze a DNA sequence to see restriction sites and map. Vector Database. Browse a digital-only collection of vector backbone information
WEBpET Expression Systems provide core reagents needed for target gene cloning and expression. • pET vector DNA, 10 µg each of the indicated plasmids • Host bacterial strains BL21, BL21(DE3) and BL21(DE3)pLysS, glycerol stocks1, 2 • …
pET Expression Vectors | Life Science Research | MilliporeSigma
WEBCollectively, our large collection of pET vector types, different host strains and companion products offer complete solutions for high level expression, purification and detection of target proteins: Browse all pET Vectors.
WEBThe pET-28a-c(+) vectors carry an N-terminal His•Tag ®/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map.
pET-28a(+) vector map and sequence - novoprolabs.com
WEBpET-28a(+) is a bacterial expression vector designed for the production of N-terminally 6xHis-tagged proteins with a thrombin cleavage site. This vector is particularly suitable for proteins that require post-translational processing to remove the His-tag.
pET-28a(+) - EcoliWiki
WEBAug 16, 2011 · The pET-28a(+) vector carries an N-terminal His•Tag/thrombin/T7•Tag configuration in addition to an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. The T7 expression region is reversed on the circular map due to the sequence numbering from the pBR322 convention.
WEBpET-28a(+) 5.4kb L a c l K a n R p B R 3 2 2 O r i f 1 O r i Blp l Bgl ll Thrombin His tag RBS Xho l Not l Hind lll Sal l Sac l EcoR l BamH l Nde l Nhe l T7 tag His tag T7 promoter lac O M C S. Bgl 11 T 7 promoter lac operator Xba 1 rbs AG ATC TCG ATC CCG CGA AAT TAA TAC GAC TCA CTA TIG GGG AAT TGT GAG CC,C, ATA …
69864 Sigma-Aldrich pET-28a(+) DNA - Novagen - Merck
WEBAll of the pET vectors and companion products are available as kits designed for convenient cloning, expression, detection, and purification of target proteins. The pET-28a-c(+) vectors carry an N-terminal His•Tag ® /thrombin/T7•Tag ® configuration plus an optional C-terminal His•Tag sequence.
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